Handbook : Unit informationEdith Cowan University

School: Science

This unit information may be updated and amended immediately prior to semester. To ensure you have the correct outline, please check it again at the beginning of semester.

Please note that given the circumstances of COVID-19, there may be some modifications to the assessment schedule promoted in Handbook for Semester 1 2020 Units. Students will be notified of all approved modifications by Unit Coordinators via email and Unit Blackboard sites. Where changes have been made, these are designed to ensure that you still meet the unit learning outcomes in the context of our adjusted teaching and learning arrangements.

• Unit Title

  Mass Spectrometry in Systems Biology

• Unit Code

  SCI6152

• Year

  2020

• Enrolment Period

  1

• Version

  1

• Credit Points

  20

• Full Year Unit

  N

• Mode of Delivery

  On Campus
  Online

• Unit Coordinator

  Dr Stacey Nichole REINKE

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Description

This unit introduces students to the application of high-resolution mass spectrometry to the systems biology fields of proteomics and metabolomics. High-resolution mass spectrometry is state-of-the-art instrumentation that is commonly used in research laboratories to comprehensively profile peptides and metabolites in biological systems. This unit covers the theory and utility of high-resolution mass spectrometry as well as the data processing workflow used to extract information from raw spectral files and convert it into a data matrix used for subsequent data analysis.

Learning Outcomes

On completion of this unit students should be able to:

1. Apply the theory of high-resolution mass spectrometry in the context of proteomics and metabolomics.
2. Critically assess methods and guidelines for mass spectral standard operating procedures, data pre-processing, feature identification, and quality assessment, as they relate to proteomics and metabolomics.
3. Pre-process mass spectral data, optimise settings for pre-processing, perform feature identification, and assess resulting data quality.
4. Defend decisions for choice of pre-processing approach, feature identification, and data cleaning.

Unit Content

1. Introduction to proteomics and metabolomics as fields of systems biology.
2. Theory of high-resolution mass spectrometry as it relates to proteomics and metabolomics.
3. Spectral pre-processing.
4. Feature identification.
5. Quality assurance, quality control, and reporting.

Learning Experience

ON-CAMPUS

Students will attend on campus classes as well as engage in learning activities through ECU Blackboard.

For more information see the Semester Timetable

ONLINE

Students will engage in learning experiences through ECU Blackboard as well as additional ECU learning technologies.

Additional Learning Experience Information

Students will work closely with staff in an industry-sponsored laboratory setting to gain first-hand experience of the theory and utility of high-resolution mass spectrometry and associated data processing workflows.

Assessment

GS1 GRADING SCHEMA 1 Used for standard coursework units

Students please note: The marks and grades received by students on assessments may be subject to further moderation. All marks and grades are to be considered provisional until endorsed by the relevant Board of Examiners.

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