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  • Michelle Colgrave

    Professor Michelle Colgrave

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Professor Michelle Colgrave

Professor of Food and Agriculture

Staff Member Details
Email: m.colgrave@ecu.edu.au
Campus: Joondalup  
ORCID iD: https://orcid.org/0000-0001-8463-805X

Michelle Colgrave is a Professor of Food and Agricultural Proteomics in the School of Science. Michelle is using proteomics, the study of proteins, using mass spectrometry (MS), to help identify key proteins that will benefit Australia's food and agriculture industries and improve human health.

Professor Michelle Colgrave joined ECU in October 2018. She has a joint appointment at CSIRO as a Principal Research Scientist in the field of proteomics and leads CSIRO's Future Protein Mission. She is also a chief investigator on the ARC Centre of Excellence for Innovations in Peptide and Protein Science (CIPPS).

Professor Colgrave’s contributions include the identification of novel proteins, characterisation of their function, as well as development of MS-based quantitative assays for food and beverage products. Professor Colgrave has focused on the application of MS as an agricultural research tool: (1) detection of differential protein and peptide expression; (2) natural product discovery leading to the development of novel pest control agents, i.e. the cyclotides; (3) large-scale proteome analysis that enables systems biology approaches to investigate environmental and/or genetic effects on plant production; (4) targeted protein quantitation, for example the measurement of gluten proteins or allergens in food and beverages.

Professor Colgrave was sole editor of the 2017 book “Proteomics in Food Science: From Farm to Fork (Elsevier) is aimed at introducing the application of MS and proteomics to the food science and agricultural research communities

Background

  • 2018                Professor, Edith Cowan University
  • 2007 – 2018    Principal Senior Research Scientist, CSIRO Agriculture and Food
  • 2006 – 2007    Senior Field Application Specialist, Applied Biosystems
  • 2002 – 2006    Postdoctoral Research Fellow, The University of Queensland

Professional Memberships

  • Association of Official Analytical Chemists (AOAC International)
  • Australasian Proteomics Society (APS)
  • Australian and New Zealand Society for Mass Spectrometry (ANZSMS)
  • American Society for Mass Spectrometry (ASMS)

Awards and Recognition

National and International Awards

  • 2021 - Vice-Chancellor’s Excellence in Research, Edith Cowan University
  • 2021 - Bowie Medal, Australian and New Zealand Society for Mass Spectrometry (ANZSMS)
  • 2020 - Australian Academy of Technology & Engineering: ICM Agrifood Award for “for Major breakthroughs in the analysis of gluten”.
  • 2017 - Runner-up “Women in Research Award”, Scopus Researcher Awards
  • 2017 - Agriculture & Food Director’s Award for Leadership

Qualifications

  • Doctor of Philosophy, University of Wollongong, 2002.
  • Bachelor of Science (Honours), University of Wollongong, 1999.

Research Outputs

2024

Journal Articles

  • Ni, D., Nelis, JL., Dawson, AL., Bourne, N., Juliano, P., Colgrave, M., Juhasz, A., Bose, U. (2024). Application of near-infrared spectroscopy and chemometrics for the rapid detection of insect protein adulteration from a simulated matrix. Food Control, 159(2024), article number 110268. https://doi.org/10.1016/j.foodcont.2023.110268.
  • Khedr, T., Gao, L., Kamphuis, LG., Bose, U., Juhasz, A., Colgrave, M. (2024). Evaluation of alkaloid levels in commercial and wild genotypes of narrow-leafed lupin. Journal of Food Composition and Analysis, 135(2024), article number 106600. https://doi.org/10.1016/j.jfca.2024.106600.
  • Bahmani, M., Juhasz, A., Bose, U., Nye-Wood, M., Blundell, M., Howitt, CA., Colgrave, M. (2024). From grain to malt: Tracking changes of ultra-low-gluten barley storage proteins after malting. Food Chemistry, 432(2024), article number 137189. https://doi.org/10.1016/j.foodchem.2023.137189.
  • Nye-Wood, M., Colgrave, M. (2024). LC-MS/MS Reveals Hordeins Are Enriched in Brewers’ Spent Grain. Journal of the American Society for Mass Spectrometry, 35(3), 409-412. https://doi.org/10.1021/jasms.3c00451.
  • O'Lone, C., Juhasz, A., Nye-Wood, M., Moody, D., Dunn, H., Ral, J., Colgrave, M. (2024). Advancing Sustainable Malting Practices: Aquaporins as Potential Breeding Targets for Improved Water Uptake during Controlled Germination of Barley (Hordeum vulgare L.). Journal of Agricultural and Food Chemistry, 72(17), 10149-10161. https://doi.org/10.1021/acs.jafc.4c00884.
  • Ahmed, T., Juhasz, A., Bose, U., Shiferaw Terefe, N., Colgrave, M. (2024). Research trends in production, separation, and identification of bioactive peptides from fungi – A critical review. Journal of Functional Foods, 119(2024), article number 106343. https://doi.org/10.1016/j.jff.2024.106343.
  • Santoso, T., Ho, TM., Vinothsankar, G., Jouppila, K., Chen, T., Owens, A., Lazarjani, MP., Farouk, MM., Colgrave, M., Otter, D., Kam, R., Le, TT. (2024). Effects of Laccase and Transglutaminase on the Physicochemical and Functional Properties of Hybrid Lupin and Whey Protein Powder. Foods, 13(13), article number 2090. https://doi.org/10.3390/foods13132090.
  • Karnaneedi, S., Johnston, EB., Bose, U., Juhasz, A., Broadbent, JA., Ruethers, T., Jerry, EM., Kamath, SD., Limviphuvadh, V., Stockwell, S., Byrne, K., Clarke, D., Colgrave, M., Maurer-Stroh, S., Lopata, AL. (2024). The Allergen Profile of Two Edible Insect Species—Acheta domesticus and Hermetia illucens. Molecular Nutrition & Food Research, 68(16), article number 2300811. https://doi.org/10.1002/mnfr.202300811.

2023

Journal Articles

  • Tahmasian, A., Drew, R., Broadbent, J., Juhasz, A., Nye-Wood, M., Colgrave, M. (2023). Conventional solid-state fermentation impacts the white lupin proteome reducing the abundance of allergenic peptides. Food Chemistry, 426(TBD), article number 136622. https://doi.org/10.1016/j.foodchem.2023.136622.
  • Bahmani, M., Juhasz, A., Bose, U., Nye-Wood, M., Blundell, M., Howitt, CA., Colgrave, M. (2023). Proteome Changes Resulting From Malting In Hordein-Reduced Barley Lines. Journal of Agricultural and Food Chemistry, 2023(Article in Press), TBD. https://doi.org/10.1021/acs.jafc.3c02292.
  • Nye-Wood, M., Byrne, K., Stockwell, S., Juhasz, A., Bose, U., Colgrave, M. (2023). Low Gluten Beers Contain Variable Gluten and Immunogenic Epitope Content. Foods, 12(17), article number 3252. https://doi.org/10.3390/foods12173252.
  • Hamzelou, S., Belobrajdic, D., Broadbent, JA., Juhasz, A., Lee Chang, K., Jameson, I., Ralph, P., Colgrave, M. (2023). Utilizing proteomics to identify and optimize microalgae strains for high-quality dietary protein: a review. Critical Reviews in Biotechnology, 2023(2023), TBD. https://doi.org/10.1080/07388551.2023.2283376.
  • O'Lone, C., Juhasz, A., Nye-Wood, M., Dunn, H., Moody, D., Ral, J., Colgrave, M. (2023). Proteomic exploration reveals a metabolic rerouting due to low oxygen during controlled germination of malting barley (Hordeum vulgare L.). Frontiers in Plant Science, 14(2023), article number 1305381. https://doi.org/10.3389/fpls.2023.1305381.
  • Nelis, J., Dawson, A., Bose, U., Anderson, A., Colgrave, M., Broadbent, J. (2023). Safe food through better labelling; a robust method for the rapid determination of caprine and bovine milk allergens. Food Chemistry, 417(TBD), article number 135885. https://doi.org/10.1016/j.foodchem.2023.135885.
  • Hamzelou, S., Belobrajdic, D., Juhasz, A., Brook, H., Bose, U., Colgrave, M., Broadbent, JA. (2023). Nutrition, Allergenicity And Physicochemical Qualities Of Food-Grade Protein Extracts From Nannochloropsis Oculata. Food Chemistry, 424(2023), article number 136459. https://doi.org/10.1016/j.foodchem.2023.136459.
  • Ibitoye, J., Phan, TT., Le, DN., Dewettinck, K., Colgrave, M., Trzcinski, AP., Ly-Nguyen, B. (2023). Improving The Quality Of Set Yoghurt Using Milk Fat Globule Membrane Fragments. Journal of Food Processing and Preservation, 2023(2023), TBD. https://doi.org/10.1155/2023/6875570.
  • Khedr, T., Juhasz, A., Singh, KB., Foley, R., Nye-Wood, M., Colgrave, M. (2023). Classification Of Unexposed Potsherd Cavities By Using Deep Learning. Journal of Food Composition and Analysis, 121(2023), article number 105391. https://doi.org/10.1016/j.jfca.2023.105391.

2022

Journal Articles

  • Tahmasian, A., Juhasz, A., Broadbent, J., Nye-Wood, M., Le, T., Colgrave, M. (2022). Evaluation of the Major Seed Storage Proteins, the Conglutins, Across Genetically Diverse Narrow-Leafed Lupin Varieties. Frontiers in Nutrition, 9(1), Article number 842168. https://doi.org/10.3389/fnut.2022.842168.
  • Kamal, N., Tsardakas Renhuldt, N., Bentzer, J., Gundlach, H., Haberer, G., Juhasz, A., Lux, T., Bose, U., Tye-Din, JA., Lang, D., Van Gessel, N., Reski, R., Fu, Y., Spégel, P., Ceplitis, A., Himmelbach, A., Waters, AJ., Bekele, WA., Colgrave, M., Hansson, M., Stein, N., Mayer, KF., Jellen, EN., Maughan, PJ., Tinker, NA., Mascher, M., Olsson, O., Spannagl, M., Sirijovski, N. (2022). The mosaic oat genome gives insights into a uniquely healthy cereal crop. Nature, 606(7912), 113-119. https://doi.org/10.1038/s41586-022-04732-y.
  • Nelis, J., Rosas Da Silva, G., Ortuno, J., Tsagkaris, A., Borremans, B., Haslova, J., Colgrave, M., Elliott, C. (2022). The General Growth Tendency: A tool to improve publication trend reporting by removing record inflation bias and enabling quantitative trend analysis. PLoS One, 17(5), Article number e0268433. https://doi.org/10.1371/journal.pone.0268433.
  • Nye-Wood, M., Juhasz, A., Tanner, GJ., Colgrave, M. (2022). Digestibility of wheat alpha-amylase/trypsin inhibitors using a caricain digestive supplement. Frontiers in Nutrition, 9(10 August 2022), Article number 977206. https://doi.org/10.3389/fnut.2022.977206.
  • Tahmasian, A., Broadbent, J., Juhasz, A., Nye-Wood, M., Le, TT., Bose, U., Colgrave, M. (2022). Evaluation of protein extraction methods for in-depth proteome analysis of narrow-leafed lupin (Lupinus angustifolius) seeds. Food Chemistry, 367(Jan 2022), Article number 130722. https://doi.org/10.1016/j.foodchem.2021.130722.
  • Bahmani, M., Juhasz, A., Broadbent, J., Bose, U., Nye-Wood, M., Edwards, I., Colgrave, M. (2022). Proteome Phenotypes Discriminate the Growing Location and Malting Traits in Field-Grown Barley. Journal of Agricultural and Food Chemistry, 70(34), 10680-10691. https://doi.org/10.1021/acs.jafc.2c03816.
  • Bull, C., Belobrajdic, D., Hamzelou, S., Jones, D., Leifert, W., Ponces-Reyes, R., Shiferaw Terefe, N., Williams, G., Colgrave, M. (2022). How Healthy are Non-Traditional Dietary Proteins? The Effect of Diverse Protein Foods on Biomarkers of Human Health. Foods, 11(4), article number 528. https://doi.org/10.3390/foods11040528.
  • Nelis, J., Broadbent, J., Bose, U., Anderson, A., Colgrave, M. (2022). Targeted proteomics for rapid and robust peanut allergen quantification. Food Chemistry, 383(30 July 2022), article number 132592. https://doi.org/10.1016/j.foodchem.2022.132592.
  • Nelis, J., Bose, U., Broadbent, J., Hughes, J., Sikes, A., Anderson, A., Caron, K., Schmoelzl, S., Colgrave, M. (2022). Biomarkers and biosensors for diagnosis of pH non-compliant, dark cutting beef pre-disposition and welfare in cattle. Comprehensive Reviews in Food Science and Food Safety, 21(3), 2391-2432. https://doi.org/10.1111/1541-4337.12935.
  • Zhang, Q., Pritchard, J., Meiog, J., Byrne, K., Colgrave, M., Wang, J., Ral, J. (2022). Over-expression of a wheat late maturity alpha-amylase type 1 impact on starch properties during grain development and germination. Frontiers in Plant Science, 13(29 March 2022), article number 811728. https://doi.org/10.3389/fpls.2022.811728.
  • Bose, U., Broadbent, J., Juhasz, A., Karnaneedi, S., Johnston, E., Stockwell, S., Byrne, K., Limviphuvadh, V., Maurer-Stroh, S., Lopata, A., Colgrave, M. (2022). Comparison of protein extraction protocols and allergen mapping from black soldier fly Hermetia illucens. Journal of Proteomics, 269(30 October 2022), Article number 104724. https://doi.org/10.1016/j.jprot.2022.104724.

2021

Journal Articles

  • Escobar Correas, S., Broadbent, J., Andraszek, A., Stockwell, S., Howitt, C., Juhasz, A., Colgrave, M. (2021). Perennial ryegrass contains gluten-like proteins that could contaminate cereal crops. Frontiers in Nutrition, 8(28 July 2021), Article number 708122. https://doi.org/10.3389/fnut.2021.708122.
  • Ding, Y., Gardiner, D., Powell, J., Colgrave, M., Park, R., Kezan, K. (2021). Adaptive defence and sensing responses of host plant roots to fungal pathogen attack revealed by transcriptome and metabolome analyses. Plant, Cell and Environment, 44(12), 3756-3774. https://doi.org/10.1111/pce.14195.
  • Bose, U., Juhasz, A., Yu, R., Bahmani, M., Byrne, K., Blundell, M., Broadbent, J., Howitt, C., Colgrave, M. (2021). Proteome and Nutritional Shifts Observed in Hordein Double-Mutant Barley Lines. Frontiers in Plant Science, 12(9 September 2021), Article number 718504. https://doi.org/10.3389/fpls.2021.718504.
  • Jadhav, S., Shah, R., Karpe, A., Barlow, R., McMillan, K., Colgrave, M., Beale, D. (2021). Utilizing the Food–Pathogen Metabolome to Putatively Identify Biomarkers for the Detection of Shiga Toxin-Producing E. coli (STEC) from Spinach. Metabolites, 11(2), Article number 67. https://doi.org/10.3390/metabo11020067.
  • Zhang, Q., Pritchard, J., Mieog, J., Byrne, K., Colgrave, M., Wang, J., Ral, J. (2021). Overexpression of a wheat α-amylase type 2 impact on starch metabolism and abscisic acid sensitivity during grain germination. The Plant Journal, 108(2), 378-393. https://doi.org/10.1111/tpj.15444.
  • Larkin, P., Liu, Q., Vanhercke, T., Zhou, X., Bose, U., Broadbent, J., Colgrave, M., Ral, J., Reynolds, K., Sun, M., El Tahchy, A., Shrestha, P., Li, Z., Jobling, S., Lonergan, P., Wu, X., Yu, R., Luo, J., Howitt, C., Newberry, M. (2021). Transgenic wheat with increased endosperm lipid – impacts on grain composition and baking quality. Journal of Cereal Science, 101(September 2021), Article number 103289. https://doi.org/10.1016/j.jcs.2021.103289.
  • Bose, U., Broadbent, J., Juhasz, A., Karnaneedi, S., Johnston, E., Stockwell, S., Byrne, K., Limviphuvadh, V., Maurer-Stroh, S., Lopata, A., Colgrave, M. (2021). Protein extraction protocols for optimal proteome measurement and arginine kinase quantitation from cricket Acheta domesticus for food safety assessment. Food Chemistry, 348(30 June 2021), Article number 129110. https://doi.org/10.1016/j.foodchem.2021.129110.
  • Broadbent, J., Condina, M., Colgrave, M. (2021). Quantitative mass spectrometry-based analysis of proteins related to cattle and their products–Focus on cows’ milk beta-casein proteoforms. Methods, 186(February 2021), 112-118. https://doi.org/10.1016/j.ymeth.2020.09.011.
  • Bahmani, M., O'Lone, C., Juhasz, A., Nye-Wood, M., Dunn, H., Edwards, I., Colgrave, M. (2021). Application of mass spectrometry-based proteomics to barley research. Journal of Agricultural and Food Chemistry, 69(31), 8591-8609. https://doi.org/10.1021/acs.jafc.1c01871.
  • Nye-Wood, M., Juhasz, A., Bose, U., Colgrave, M. (2021). Proteome Analysis and Epitope Mapping in a Commercial Reduced-Gluten Wheat Product. Frontiers in Nutrition, 8(11 August), Article number 705822. https://doi.org/10.3389/fnut.2021.705822.
  • Colgrave, M., Dominik, S., Tobin, A., Stockmann, R., Simon, C., Howitt, C., Belobrajdic, D., Paull, C., Vanhercke, T. (2021). Perspectives on Future Protein Production. Journal of Agricultural and Food Chemistry, 69(50), 15076-15083. https://doi.org/10.1021/acs.jafc.1c05989.

Reports

  • Colgrave, M., Nye-Wood, M. (2021). Sensient Technologies: Cyclotide identification and quantitation. Australia. Sensient Technologies Europe GmbH,.

2020

Journal Articles

  • Allred, L., Nye-Wood, M., Colgrave, M. (2020). Analysis of Gluten in Dried Yeast and Yeast-Containing Products. Foods, 9(12), Article Number: 1790. https://doi.org/10.3390/foods9121790.
  • Juhasz, A., Colgrave, M., Howitt, CA. (2020). Developing gluten-free cereals and the role of proteomics in product safety. Journal of Cereal Science, 93(May 2020), Article number 102932. https://doi.org/10.1016/j.jcs.2020.102932.
  • Bose, U., Juhasz, A., Broadbent, JA., Komatsu, S., Colgrave, M. (2020). Multi-Omics Strategies for Decoding Smoke-Assisted Germination Pathways and Seed Vigour. International Journal of Molecular Sciences, 21(20), Article number 7512. https://doi.org/10.3390/ijms21207512.
  • Luo, J., Butardo, V., Yang, Q., Konik-Rose, C., Colgrave, M., Millar, A., Jobling, S., Li, Z. (2020). The impact of the indica rice SSIIa allele on the apparent high amylose starch from rice grain with downregulated japonica SBEIIb. Theoretical and Applied Genetics, 133(10), 2961–2974. https://doi.org/10.1007/s00122-020-03649-2.
  • Mendoza-Porras, O., Pires, P., Goswami, H., Meirelles, F., Colgrave, M., Wijffels, G. (2020). Cytokines in the grass, a lesson learnt: Measuring cytokines in plasma using multiple reaction monitoring mass spectrometry. Rapid Communications in Mass Spectrometry, 34(9), Article number e8723. https://doi.org/10.1002/rcm.8723.
  • Allen, R., Gregg, C., Okada, S., Menon, A., Hussain, D., Gillespie, V., Johnston, E., Devilla, R., Warden, A., Taylor, M., Byrne, K., Colgrave, M., Wood, C. (2020). Plant expression of NifD protein variants resistant to mitochondrial degradation. Proceedings of the National Academy of Sciences of USA, 117(37), 23165-23173. https://doi.org/10.1073/pnas.2002365117.
  • Okada, S., Gregg, C., Allen, R., Menon, A., Hussain, D., Gillespie, V., Johnston, E., Byrne, K., Colgrave, M., Wood, C. (2020). A Synthetic Biology Workflow Reveals Variation in Processing and Solubility of Nitrogenase Proteins Targeted to Plant Mitochondria, and Differing Tolerance of Targeting Sequences in a Bacterial Nitrogenase Assay. Frontiers in Plant Science, 11(10 September 2020), 552160. https://doi.org/10.3389/fpls.2020.552160.
  • Bose, U., Broadbent, J., Byrne, K., Blundell, M., Howitt, C., Colgrave, M. (2020). Proteome Analysis of Hordein-Null Barley Lines Reveals Storage Protein Synthesis and Compensation Mechanisms. Journal of Agricultural and Food Chemistry, 68(20), 5763-5775. https://doi.org/10.1021/acs.jafc.0c01410.
  • Bose, U., Juhasz, A., Broadbent, JA., Byrne, K., Howitt, CA., Colgrave, M. (2020). Identification and Quantitation of Amylase Trypsin Inhibitors across Cultivars Representing the Diversity of Bread Wheat. Journal of Proteome Research, 19(5), 2136-2148. https://doi.org/10.1021/acs.jproteome.0c00059.
  • Mendoza-Porras, O., Kamith, S., Harris, J., Colgrave, M., Huerlimann, R., Lopata, A., Wade, N. (2020). Resolving hemocyanin isoform complexity in haemolymph of black tiger shrimp Penaeus monodon - implications in aquaculture, medicine and food safety. Journal of Proteomics, 218(30 April 2020), Article number 103689. https://doi.org/10.1016/j.jprot.2020.103689.

2019

Book Chapters

  • Colgrave, M., Allingham, P., Tyrrell, K., Jones, A. (2019). Multiple Reaction Monitoring for the Accurate Quantification of Amino Acids: Using Hydroxyproline to Estimate Collagen Content. Amino Acid Analysis Methods and Protocols (33-45). Springer. https://doi.org/10.1007/978-1-4939-9639-1_4.
  • Bose, U., Wijffels, G., Howitt, C., Colgrave, M. (2019). Proteomics: Tools of the Trade. Advances in Experimental Medicine and Biology (1-22). Springer Nature. https://doi.org/10.1007/978-3-030-12298-0_1.
  • Li, H., Byrne, K., Howitt, C., Colgrave, M. (2019). Efficient extraction and digestion of gluten proteins. Functional Proteomics: Methods and Protocols (405-412). Humana Press. https://doi.org/10.1007/978-1-4939-8814-3_22.

Journal Articles

  • Botwright, N., Zhao, M., Wang, T., McWilliam, S., Colgrave, M., Hlinka, O., Li, S., Suwansa-Ard, S., Subramanian, S., McPherson, L., King, H., Reverter, A., Cook, M., McGrath, A., Elliott, N., Cummins, S. (2019). Greenlip Abalone (Haliotis laevigata) Genome and Protein Analysis Provides Insights into Maturation and Spawning. G3: Genes, Genomes, Genetics, 9(10), 3067-3078. https://doi.org/10.1534/g3.119.400388.
  • Tanner, G., Juhasz, A., Florides, CG., Nye-Wood, M., Békés, F., Colgrave, M., Russell, AK., Hardy, MY., Tye-Din, JA. (2019). Preparation and characterization of avenin-enriched oat protein by chill precipitation for feeding trials in celiac disease. Frontiers in Nutrition, 6(15 October 2019), article no.162. https://doi.org/10.3389/fnut.2019.00162.
  • Lexhaller, B., Colgrave, M., Scherf, KA. (2019). Characterization and Relative Quantitation of Wheat, Rye, and Barley Gluten Protein Types by Liquid Chromatography–Tandem Mass Spectrometry. Frontiers in Plant Science, 10(December 2019), Article number: 1530. https://doi.org/10.3389/fpls.2019.01530.
  • Li, H., Bose, U., Stockwell, S., Howitt, C., Colgrave, M. (2019). Assessing the utility of multiplexed liquid chromatography-mass spectrometry for gluten detection in Australian breakfast food products. Molecules, 24(20), Article Number 3665. https://doi.org/10.3390/molecules24203665.
  • Colgrave, M., Byrne, K., Pillai, S., Dong, B., Leonforte, A., Caine, J., Kowalczyk, L., Scoble, J., Petrie, J., Singh, S., Zhou, X. (2019). Quantitation of seven transmembrane proteins from the DHA biosynthesis pathway in genetically engineered canola by targeted mass spectrometry. Food and Chemical Toxicology, 126(April 2019), 313-321. https://doi.org/10.1016/j.fct.2019.02.035.
  • Bose, U., Broadbent, J., Byrne, K., Hasan, S., Howitt, C., Colgrave, M. (2019). Optimisation of protein extraction for in-depth profiling of the cereal grain proteome. Journal of Proteomics, 197(15 April 2019), 23-33. https://doi.org/10.1016/j.jprot.2019.02.009.
  • Escobar Correas, S., Mendoza-Porras, O., Dellagnola, F., Colgrave, M., Vega, I. (2019). Integrative Proteomic Analysis of Digestive Tract Glycosidases from the Invasive Golden Apple Snail, Pomacea canaliculata. Journal of Proteome Research, 18(9), 3342-3352. https://doi.org/10.1021/acs.jproteome.9b00282.
  • Colgrave, M., Byrne, K., Caine, J., Kowalczyk, L., Vibhakaran Pillai, S., Dong, B., Lovrecz, G., Macintosh, S., Scoble, J., Petrie, J., Singh, S., Zhou, X. (2019). Proteomics reveals the in vitro protein digestibility of seven transmembrane enzymes from the docosahexaenoic acid biosynthesis pathway. Food and Chemical Toxicology, 130(August 2019), 89-98. https://doi.org/10.1016/j.fct.2019.05.015.
  • Bose, U., Byrne, K., Howitt, C., Colgrave, M. (2019). Targeted proteomics to monitor the extraction efficiency and levels of barley α-amylase trypsin inhibitors that are implicated in non-coeliac gluten sensitivity. Journal of Chromatography A, 1600(30 August 2019), 55-64. https://doi.org/10.1016/j.chroma.2019.04.043.
  • Pasquali, D., Blundell, M., Howitt, C., Colgrave, M. (2019). Catcher of the Rye: Detection of Rye, a Gluten-Containing Grain, by LC-MS/MS. Journal of Proteome Research, 18(9), 3394-3403. https://doi.org/10.1021/acs.jproteome.9b00314.
  • Tanner, GJ., Colgrave, M., Blundell, M., Howitt, C., Bacic, A. (2019). Hordein Accumulation in Developing Barley Grains. Frontiers in Plant Science, 10(2019), Article number 649. https://doi.org/10.3389/fpls.2019.00649.

2018

Journal Articles

  • Jamtgard, S., Robinson, N., Moritz, T., Colgrave, M., Schmidt, S. (2018). Optimising methods for the recovery and quantification of di- and tripeptides in soil. Soil Research, 56(4), 404-412. https://doi.org/10.1071/SR17279.
  • Niyomploy, P., Chan, L., Harvey, P., Poth, A., Colgrave, M., Craik, D. (2018). Discovery and Characterization of Cyclotides from Rinorea Species. Journal of Natural Products, 81(11), 2512-2520. https://doi.org/10.1021/acs.jnatprod.8b00572.
  • Deatley, K., Colgrave, M., Canovas, A., Wijffels, G., Ashley, R., Silver, G., Rincon, G., Medrano, J., Islas-Trejo, A., Fortes, M., Reverter, A., Porto-Neto, L., Lehnert, S., Thomas, M. (2018). Neuropeptidome of the Hypothalamus and Pituitary Gland of Indicine × Taurine Heifers: Evidence of Differential Neuropeptide Processing in the Pituitary Gland before and after Puberty. Journal of Proteome Research, 17(5), 1852-1865. https://doi.org/10.1021/acs.jproteome.7b00875.
  • Li, H., Byrne, K., Galiamov, R., Mendoza-Porras, O., Bose, U., Howitt, C., Colgrave, M. (2018). Using LC-MS to examine the fermented food products vinegar and soy sauce for the presence of gluten. Food Chemistry, 254(15 July 2018), 302-308. https://doi.org/10.1016/j.foodchem.2018.02.023.
  • Dawsonl, C., Mendoza-Porras, O., Byrne, K., Hooper, T., Howitt, C., Colgrave, M. (2018). Oat of this world: Defining peptide markers for detection of oats in processed food. Peptide Science, 110(3), Article no.e24045. https://doi.org/10.1002/pep2.24045.

2017

Journal Articles

  • Franke, B., James, A., Mobli, M., Colgrave, M., Mylne, J., Rosengren, K. (2017). Two proteins for the price of one: Structural studies of the dual-destiny protein preproalbumin with sunflower trypsin inhibitor-1. Journal of Biological Chemistry, 292(30), 12398-12411. https://doi.org/10.1074/jbc.M117.776955.
  • Fallabaghery, A., Zou, W., Byrne, K., Howitt, C., Colgrave, M. (2017). Comparison of Gluten Extraction Protocols Assessed by LC-MS/MS Analysis. Journal of Agricultural and Food Chemistry, 65(13), 2857-2866. https://doi.org/10.1021/acs.jafc.7b00063.
  • Colgrave, M., Byrne, K., Howitt, C. (2017). Food for thought: Selecting the right enzyme for the digestion of gluten. Food Chemistry, 234(Nov), 389-397. https://doi.org/10.1016/j.foodchem.2017.05.008.
  • Colgrave, M., Byrne, K., Howitt, C. (2017). Liquid Chromatography-Mass Spectrometry Analysis Reveals Hydrolyzed Gluten in Beers Crafted to Remove Gluten. Journal of Agricultural and Food Chemistry, 65(44), 9715-9725. https://doi.org/10.1021/acs.jafc.7b03742.
  • Mendoza-Porras, O., Botwright, N., Reverter, A., Cook, M., Harris, J., Wijffels, G., Colgrave, M. (2017). Identification of differentially expressed reproductive and metabolic proteins in the female abalone (Haliotis laevigata) gonad following artificial induction of spawning. Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 24(December 2017), 127-138. https://doi.org/10.1016/j.cbd.2016.04.005.
  • Powell, J., Carere, J., Sablok, G., Fitzgerald, T., Stiller, J., Colgrave, M., Gardiner, D., Manners, J., Vogel, J., Henry, R., Kazan, K. (2017). Transcriptome analysis of Brachypodium during fungal pathogen infection reveals both shared and distinct defense responses with wheat. Scientific Reports, 7(1), Article no.17212. https://doi.org/10.1038/s41598-017-17454-3.
  • Nix, A., Paull, C., Colgrave, M. (2017). Flavonoid profile of the cotton plant, Gossypium hirsutum: A review. Plants, 6(4), article no.43. https://doi.org/10.3390/plants6040043.
  • Ravipati, A., Poth, A., Troeira Henriques, S., Bhandari, M., Huang, Y., Nino, J., Colgrave, M., Craik, D. (2017). Understanding the Diversity and Distribution of Cyclotides from Plants of Varied Genetic Origin. Journal of Natural Products, 80(5), 1522-1530. https://doi.org/10.1021/acs.jnatprod.7b00061.
  • Powell, J., Carere, J., Fitzgerald, T., Stiller, J., Covarelli, L., Xu, Q., Gubler, F., Colgrave, M., Gardiner, D., Manners, J., Henry, R., Kazan, K. (2017). The Fusarium crown rot pathogen Fusarium pseudograminearum triggers a suite of transcriptional and metabolic changes in bread wheat (Triticum aestivum L.). Annals of Botany, 119(5), 853-867. https://doi.org/10.1093/aob/mcw207.
  • Mendoza-Porras, O., Harris, J., Wijffels, G., Reverter, A., Cook, M., Botwright, N., Colgrave, M. (2017). Gonadal reproductive and metabolic proteins of male abalone Haliotis laevigata (Donovan, 1808) assessed by targeted mass spectrometry after artificial induction of spawning. Aquaculture Research, 48(12), 6009-6015. https://doi.org/10.1111/are.13413.

2016

Journal Articles

  • Pinto, M., Silva, O., Viana, J., Porto, W., Migliolo, L., De Cunha, N., Gomes, N., Fensterseifer, I., Colgrave, M., Craik, D., Dias, S., Franco, O. (2016). Characterization of a Bioactive Acyclotide from Palicourea rigida. Journal of Natural Products, 79(11), 2767-2773. https://doi.org/10.1021/acs.jnatprod.6b00270.
  • Carere, J., Colgrave, M., Stiller, J., Liu, C., Manners, J., Kazan, K., Gardiner, D. (2016). Enzyme-driven metabolomic screening: a proof-of-principle method for discovery of plant defence compounds targeted by pathogens. New Phytologist, 212(3), 770-779. https://doi.org/10.1111/nph.14067.
  • Niyomploy, P., Chan, L., Poth, A., Colgrave, M., Sangvanich, P., Craik, D. (2016). Discovery, isolation, and structural characterization of cyclotides from Viola sumatrana Miq. Biopolymers, 106(6), 796-805. https://doi.org/10.1002/bip.22914.
  • Colgrave, M., Byrne, K., Blundell, M., Howitt, C. (2016). Identification of barley-specific peptide markers that persist in processed foods and are capable of detecting barley contamination by LC-MS/MS. Journal of Proteomics, 147(Sep 2016), 169-176. https://doi.org/10.1016/j.jprot.2016.03.045.
  • Franke, B., Colgrave, M., Mylne, J., Rosengren, K. (2016). Mature forms of the major seed storage albumins in sunflower: A mass spectrometric approach. Journal of Proteomics, 147(Sep 2016), 177-186. https://doi.org/10.1016/j.jprot.2016.05.004.
  • Yu, X., James, A., Yang, A., Jones, A., Mendoza-Porras, O., Bétrix, C., Ma, H., Colgrave, M. (2016). A comparative proteomic study of drought-tolerant and drought–sensitive soybean seedlings under drought stress. Crop and Pasture Science, 67(5), 528-540. https://doi.org/10.1071/CP15314.
  • Tanner, G., Blundell, M., Colgrave, M., Howitt, C. (2016). Creation of the first ultra-low gluten barley (Hordeum vulgare L.) for coeliac and gluten-intolerant populations. Plant Biotechnology Journal, 14(4), 1139-1150. https://doi.org/10.1111/pbi.12482.
  • Colgrave, M., Byrne, K., Blundell, M., Heidelberger, S., Lane, C., Tanner, G., Howitt, C. (2016). Comparing Multiple Reaction Monitoring and Sequential Window Acquisition of All Theoretical Mass Spectra for the Relative Quantification of Barley Gluten in Selectively Bred Barley Lines. Analytical Chemistry, 88(18), 9127-9135. https://doi.org/10.1021/acs.analchem.6b02108.

2015

Journal Articles

  • Himeno, K., Rosengren, K., Inoue, T., Perez, R., Colgrave, M., Lee, H., Chan, L., Henriques, S., Fujita, K., Ishibashi, N., Zendo, T., Wilaipun, P., Nakayama, J., Leelawatcharamas, V., Jikuya, H., Craik, D., Sonomoto, K. (2015). Identification, Characterization, and Three-Dimensional Structure of the Novel Circular Bacteriocin, Enterocin NKR-5-3B, from Enterococcus faecium. Biochemistry, 54(31), 4863-4876. https://doi.org/10.1021/acs.biochem.5b00196.
  • Luo, J., Ahmed, R., Kosar-Hashemi, B., Larroque, O., Butardo, V., Tanner, G., Colgrave, M., Upadhyaya, N., Tetlow, I., Emes, M., Millar, A., Jobling, S., Morell, M., Li, Z. (2015). The different effects of starch synthase IIa mutations or variation on endosperm amylose content of barley, wheat and rice are determined by the distribution of starch synthase I and starch branching enzyme IIb between the starch granule and amyloplast stroma. Theoretical and Applied Genetics: international journal of plant breeding research, 128(7), 1407-1419. https://doi.org/10.1007/s00122-015-2515-z.
  • Nix, A., Paull, C., Colgrave, M. (2015). The flavonoid profile of pigeonpea, Cajanus cajan: a review. SpringerPlus, 4(1), 6p.. https://doi.org/10.1186/s40064-015-0906-x.
  • Ravipati, A., Henriques, S., Poth, A., Kaas, Q., Wang, C., Colgrave, M., Craik, D. (2015). Lysine-rich Cyclotides: A New Subclass of Circular Knotted Proteins from Violaceae. ACS Chemical Biology, 10(11), 2491−2500. https://doi.org/10.1021/acschembio.5b00454.
  • Colgrave, M., Goswami, H., Byrne, K., Blundell, M., Howitt, C., Tanner, G. (2015). Proteomic Profiling of 16 Cereal Grains and the Application of Targeted Proteomics To Detect Wheat Contamination. Journal of Proteome Research, 14(6), 2659-2668. https://doi.org/10.1021/acs.jproteome.5b00187.

2014

Journal Articles

  • Whan, A., Dielen, A., Mieog, J., Bowerman, A., Robinson, H., Byrne, K., Colgrave, M., Larkin, P., Howitt, C., Morell, M., Ral, J. (2014). Engineering α-amylase levels in wheat grain suggests a highly sophisticated level of carbohydrate regulation during development. Journal of Experimental Botany, 65(18), 5443–5457. https://doi.org/10.1093/jxb/eru299.
  • Mendoza-Porras, O., Botwright, N., McWilliam, S., Cook, M., Harris, J., Wijffels, G., Colgrave, M. (2014). Exploiting genomic data to identify proteins involved in abalone reproduction. Journal of Proteomics, 108(28 August 2014), 337-353. https://doi.org/10.1016/j.jprot.2014.06.001.
  • Canovas, A., Reverter, A., Deatley, K., Ashley, R., Colgrave, M., Fortes, M., Islas-Trejo, A., Lehnert, S., Porto-Neto, L., Rincon, G., Silver, G., Snelling, W., Medrano, J., Thomas, M. (2014). Multi-Tissue Omics Analyses Reveal Molecular Regulatory Networks for Puberty in Composite Beef Cattle. PLoS One, 9(7), Article number e102551. https://doi.org/10.1371/journal.pone.0102551.
  • Elliott, A., Delay, C., Liu, H., Phua, Z., Rosengren, J., Benfield, A., Panero, J., Colgrave, M., Jayasena, A., Dunse, K., Anderson, M., Schilling, E., Ortiz-Barrientos, D., Craik, D., Mylne, J. (2014). Evolutionary Origins of a Bioactive Peptide Buried within Preproalbumin. The Plant Cell, 26(3), 981-995. https://doi.org/10.1105/tpc.114.123620.
  • Osborne, S., Chen, W., Addepalli, R., Colgrave, M., Singh, T., Tran, C., Day, L. (2014). In vitro transport and satiety of beta-lactoglobulin dipeptide and beta-casomorphin-7 and its metabolites. Food and Function, 5(11), 2706-2718. https://doi.org/10.1039/c4fo00164h.
  • Menzies, M., Seim, I., Josh, P., Nagaraj, S., Lees, M., Walpole, C., Chopin, L., Colgrave, M., Ingham, A. (2014). Cloning and tissue distribution of novel splice variants of the ovine ghrelin gene. BMC Veterinary Research, 10(1), Article No. 211. https://doi.org/10.1186/s12917-014-0211-x.
  • Colgrave, M., Goswami, H., Blundell, M., Howitt, C., Tanner, G. (2014). Using mass spectrometry to detect hydrolysed gluten in beer that is responsible for false negatives by ELISA. Journal of Chromatography A, 1370(28 November 2014), 105-114. https://doi.org/10.1016/j.chroma.2014.10.033.

Research Projects

  • Development of singular and modular multi-purpose peptides for efficient crop protection  , Commonwealth Scientific and Industrial Research Organisation (CSIRO), Industry PhD (iPhD) Program, 2024 ‑ 2029, $194,511.
  • Program 2: Discovery of desirable aroma and flavour compounds for oat food/beverage applications, Grains Research & Development Corporation, Tender, 2024 ‑ 2028, $525,656.
  • CSP2403-018RTX Program 2: Enhancing the agronomic and nutritive value of narrow-leafed lupin by reducing seed coat thickness and increasing protein content, Grains Research & Development Corporation, Grant, 2024 ‑ 2028.
  • ARC Centre of Excellence for Innovations in Peptide and Protein Science, Australian Research Council, Grant - Centres of Excellence (CEE126), 2020 ‑ 2027, $1,818,376.
  • Machine Learning Identification of Bioactive Peptides from Australian Food Crops, Commonwealth Scientific and Industrial Research Organisation (CSIRO), Top Up Scholarship, 2023 ‑ 2026, $57,500.
  • Decoding germination defects that threaten global wheat production, Australian Research Council, Grant - Discovery Projects, 2021 ‑ 2025, $523,288.
  • Proteomic characterisation of malting barley varieties, Commonwealth Scientific and Industrial Research Organisation (CSIRO), Industry PhD (iPhD) Program, 2020 ‑ 2024, $132,000.
  • Can some common gluten free foods behave like gluten? An investigation into a novel cause of persistently symptomatic or active coeliac disease, Coeliac Australia, Research Project Grant, 2021 ‑ 2024, $163,636.
  • Understanding the impact of fertiliser use on the nutritional quality of oat, Edith Cowan University, Early-Mid Career Researcher Grant Scheme 2022 (Stream 2), 2023 ‑ 2024, $39,768.
  • Non-responsive Coeliac Disease – Understanding the Grass Roots Cause for Gluten Free Diet Failure, Commonwealth Scientific and Industrial Research Organisation (CSIRO), CSIRO - Fellowship, 2020 ‑ 2023, $381,361.
  • Characterization of major allergens in white lupin, Eighth Day Foods, Grant, 2022, $7,200.
  • Sensient Technologies: Cyclotide identification and quantitation (Phase 2), Sensient Technologies Europe GmbH, Grant, 2022, $16,500.
  • Lupin: prospective superfood or potential allergen, Commonwealth Scientific and Industrial Research Organisation (CSIRO), CSIRO - Scholarship, 2020 ‑ 2022, $35,750.
  • Application of proteomics methods to monitor the ability of GluteGuard to detoxify wheat gluten, Glutagen Pty Ltd, Grant, 2021, $50,440.
  • Sensient Technologies: Cyclotide identification and quantitation, Sensient Technologies Europe GmbH, Grant, 2021, $25,000.
  • Application of proteomics methods to monitor the ability of GluteGuard to detoxify ATIs, Edith Cowan University, ECU Industry Engagement Grant, 2019 ‑ 2020, $89,970.

Research Student Supervision

Current Supervisions

Principal Supervisor

  • Doctor of Philosophy, Identification and Characterisation of Bioactive Peptides in Koala (Phascolarctos cinereus) Milk
  • Doctor of Philosophy, Understanding the ultrasound-assisted hydrolysis and fermentation process for fungal enzyme and bioactive peptide production

Associate Supervisor

  • Doctor of Philosophy, Identification and characterisation of bioactive peptides in selected Cucurbitaceae crops
  • Doctor of Philosophy, Combining mass spectrometry and machine learning to discover bioactive peptides from crops
  • Doctor of Philosophy, Characterising oat grain protein quality requirements for oat milk using mass spectrometry-based proteomics

Completed Supervisions

Principal Supervisor

  • Doctor of Philosophy, Lupin: Prospective superfood or potential allergen?
  • Doctor of Philosophy, Proteomic understanding of grain germination to improve the selection of barley varieties with internationally competitive malting quality characteristics.
  • Doctor of Philosophy, Mass spectrometry-based proteomic analysis to characterise barley breeding lines
  • Doctor of Philosophy, Bitter sweet: Exploring alkaloid synthesis in lupin using LC-MS/MS

Associate Supervisor

  • Doctor of Philosophy: Proteomics as a tool for rapid detection and characterisation of cyclic peptides: a path to discoveries in cyclotide biosynthesis and evolution
  • Doctor of Philosophy: The mode of action of cyclotides: Functional studies of the prototypic cyclotide kalata B1
  • Doctor of Philosophy: Molecular Assessment of Spawning Cues in Temperate Abalone Haliotis laevigata
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